Please cite as:

CRISPRscan: Designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo: Miguel A. Moreno-Mateos*, Charles E. Vejnar*, Jean-Denis Beaudoin, Juan P. Fernandez, Emily K. Mis, Mustafa K. Khokha and Antonio J. Giraldez; *Equal contribution; Nature Methods 2015; doi:10.1038/nmeth.3543; PMID:26322839

Please cite, if you use…

Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes: Jeremy Vicencio*, Carlos Sánchez-Bolaños*, Ismael Moreno-Sánchez, David Brena, Charles E. Vejnar, Dmytro Kukhtar, Miguel Ruiz-López, Mariona Cots-Ponjoan, Alejandro Rubio, Natalia Rodrigo Melero, Jesús Crespo-Cuadrado, Carlo Carolis, Antonio J. Pérez-Pulido, Antonio J. Giraldez, Benjamin P. Kleinstiver, Julián Cerón, Miguel A. Moreno-Mateos; *Equal contribution; Nature Communications 2022; https://doi.org/10.1038/s41467-022-30228-4; PMID:35552388

CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing: Miguel A. Moreno-Mateos, Juan P. Fernandez, Romain Rouet, Charles E. Vejnar, Maura A. Lane, Emily Mis, Mustafa K. Khokha, Jennifer A. Doudna, Antonio J. Giraldez; Nature Communications 2017; doi:10.1038/s41467-017-01836-2; PMID:29222508

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation: John G. Doench, Ella Hartenian, Daniel B. Graham, Zuzana Tothova, Mudra Hegde, Ian Smith, Meagan Sullender, Benjamin L. Ebert, Ramnik J. Xavier, and David E. Root; Nature Biotechnology 2014; doi:10.1038/nbt.3026; PMID:25184501